TRANSGLYCOSYLATION ACTIVITY AND CHARACTERIZATION OF RECOMBINANT SUCROSE PHOSPHORYLASE FROM LEUCONOSTOC MESENTEROIDES MBFWRS-3(1) EXPRESSED IN ESCHERICHIA COLI
نویسندگان
چکیده
منابع مشابه
The fermentation of sucrose by Leuconostoc mesenteroides.
The slime forming Leuconostoc mesnreroides is distributed widely in nature and is apparently ubiquitous in sugar factories. Neill, Hehre, Sugg, and Jaffe (1939) found dextran, a fermentation product of L. meenteroides, in all samples of cp sucrose examined. This organism, long a nuisance in the factory, has in recent years attracted wide interest because of the commercial possibilities of the d...
متن کاملPreparation and characterization of dextran from Leuconostoc mesenteroides.
In conjunction with investigations on the a-l, 6-glucosidic linkage in starch, it was desirable to conduct comparative studies on other substances containing this linkage. The dextran from Leuconostoc mesenteroides, a polysaccharide having predominantly a-1 ,6-glucosidic linkages, was selected to be used directly in these comparative studies and to provide a source of simpler substances contain...
متن کاملEthanol synthesis from glycerol by Escherichia coli redox mutants expressing adhE from Leuconostoc mesenteroides.
AIMS Analysis of the physiology and metabolism of Escherichia coli arcA and creC mutants expressing a bifunctional alcohol-acetaldehyde dehydrogenase from Leuconostoc mesenteroides growing on glycerol under oxygen-restricted conditions. The effect of an ldhA mutation and different growth medium modifications was also assessed. METHODS AND RESULTS Expression of adhE in E. coli CT1061 [arcA cre...
متن کاملPURIFICATION AND CHARACTERIZATION OF THE CLONED HUMAN GM-CSF GENE EXPRESSED IN ESCHERICHIA COLI
The human granulocyte-macrophage colony stimulation factor (hGM-CSF) gene was cloned in the pET 23a( +) expression vector under the control of strong bacteriophage T7 transcription and translation signals. The hGM-CSF gene was transferred into E. coli strainBL21 (DE3)pLysS andIPTG was used for induction of GM-CSF gene. Production of the target protein was obtained as revealed by ELISA and ...
متن کاملPurification and Characterization of Glucosyltransferases from New Strains Leuconostoc Mesenteroides
The Leuconostoc species require sucrose in the culture medium as an inducer for the elaboration of glucosyltransferase (GTF) and glucans. Among all the reported putification methods, polyethylene glycol (PEG) is a effective and single step purification method for GTF from Leuconostoc mesenteroides. The extracellular GTFs from Leuconostoc mesenteroides L.m.28 and mutant Leuconostoc mesenteroides...
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ژورنال
عنوان ژورنال: International Journal of Applied Pharmaceutics
سال: 2020
ISSN: 0975-7058
DOI: 10.22159/ijap.2020.v12s1.ff058